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- Title
Identification of a functional toxin-antitoxin system located in the genomic island PYG1 of piezophilic hyperthermophilic archaeon <italic>Pyrococcus yayanosii</italic>.
- Authors
Li, Zhen; Song, Qinghao; Wang, Yinzhao; Xiao, Xiang; Xu, Jun
- Abstract
Toxin-antitoxin (TA) system is bacterial or archaeal genetic module consisting of toxin and antitoxin gene that be organized as a bicistronic operon. TA system could elicit programmed cell death, which is supposed to play important roles for the survival of prokaryotic population under various physiological stress conditions. The phage abortive infection system (AbiE family) belongs to bacterial type IV TA system. However, no archaeal AbiE family TA system has been reported so far. In this study, a putative AbiE TA system (PygAT), which is located in a genomic island PYG1 in the chromosome of <italic>Pyrococcus yayanosii</italic> CH1, was identified and characterized. In <italic>Escherichia coli</italic>, overexpression of the toxin gene <italic>pygT</italic> inhibited its growth while the toxic effect can be suppressed by introducing the antitoxin gene <italic>pygA</italic> in the same cell. PygAT also enhances the stability of shuttle plasmids with archaeal plasmid replication protein Rep75 in <italic>E. coli.</italic> In <italic>P. yayanosii</italic>, disruption of antitoxin gene <italic>pygA</italic> cause a significantly growth delayed under high hydrostatic pressure (HHP). The antitoxin protein PygA can specifically bind to the PygAT promoter region and regulate the transcription of <italic>pygT</italic> gene in vivo. These results show that PygAT is a functional TA system in <italic>P. yayanosii</italic>, and also may play a role in the adaptation to HHP environment.
- Subjects
ANTITOXINS; GLYCOGEN phosphorylase; BAROPHILIC bacteria; ARCHAEBACTERIA genetics; THERMOPHILIC archaebacteria; PYROCOCCUS
- Publication
Extremophiles, 2018, Vol 22, Issue 3, p347
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-018-1002-2