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- Title
Porcine peroxisome proliferator-activated receptor δ mediates the lipolytic effects of dietary fish oil to reduce body fat deposition.
- Authors
Yu, Y. H.; Wang, P. H.; Cheng, W. T. K.; Mersmann, H. J.; Wu, S. C.; Ding, S. T.
- Abstract
Peroxisome proliferator–activated receptor ö promotes fatty acid catabolism and energy expenditure in skeletal muscle and adipose tissues. A ligand for PPARδ is required to activate PPAR function. Polyunsaturated fatty acids are potential ligands for PPARδ activation. The current experiment was designed to determine the potential for PUFA, particularly from dietary fish oil, to activate porcine PPARδ in vivo. Transgenic mice were generated to overexpress porcine PPARδ in the adipose tissue. Mice were fed a high–saturated fat (13% beef tallow), or high–unsaturated fat (13% fish oil) diet, or a diet containing 4 mg/ kg of a PPAR6 ligand (L165041) for 4 mo. Compared with beef tallow feeding, fish oil feeding reduced fat mass and decreased (P < 0.05) plasma triacyiglycerol and FFA concentrations in the transgenic mice. Adipose tissue expression of genes involved in adipogenesis (i.e., lipoprotein lipase and adipocyte fatty acid–binding protein) was decreased in transgenic mice fed fish oil or the PPAR6 ligand. In the same mice, expression of the lipolytic gene, hormone–sensitive lipase was increased (P < 0.05). Fish oil feeding also stimulated expression of genes participating in fatty acid oxidation in the liver of transgenic mice compared with wild–type mice. Overall, these results indicate that PUFA may serve as natural and effective regulators of lipid catabolism in vivo and many of these effects may be generated from activation of PPARδ.
- Subjects
PEROXISOMES; ADIPOSE tissues; UNSATURATED fatty acids; LIPID metabolism; FISH oils; GENE expression; TRANSGENIC mice; LIPOPROTEIN lipase; FAT cells
- Publication
Journal of Animal Science, 2010, Vol 88, Issue 6, p2009
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2009-2597