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- Title
Docosahexaenoic acid regulates adipogenic genes in myoblasts via porcine peroxisome proliferator-activated receptor γ.
- Authors
Yu, Y. H.; Lin, E. C.; Wu, S. C.; Cheng, W. T. K.; Mersmann, H. J.; Wang, P. H.; Ding, S. T.
- Abstract
The nuclear transcription factor peroxisome proliferator-activated receptor γ (PPAR-γ) triggers adipocyte differentiation by regulating lipogenic genes. A ligand for PPARγ is necessary to activate PPARγ function. Fatty acids are potential ligands for PPARγ activation. The current experiment was designed to determine the potential for individual fatty acids to activate porcine PPARγ ectopically expressed in myoblasts. The expression of adipocyte fatty acid binding protein (aP2) and adiponectin in myoblasts stably expressing porcine PPARγ was increased when docosahexaenoic acid (DHA) was added to the adipogenic medium. The response was positively related to DHA concentration and suggests that DHA may bind to and activate porcine PPARγ, leading to increased expression of aP2 and adiponectin. The conditioned media collected from myoblasts expressing PPARγ between d 3 and 6 or between d 6 and 9, but not DHA itself, activated the aP2 gene promoter-driven luciferase activity. These results suggest that a metabolite of DHA is the ligand binding to and activating porcine PPARγ. The metabolite and pathway for its production are currently unknown.
- Subjects
SWINE growth; DOCOSAHEXAENOIC acid; TRANSCRIPTION factors; PORCINE somatotropin; CARRIER proteins; MYOBLASTS; METABOLITES; TOXIN receptors; LIGANDS (Biochemistry)
- Publication
Journal of Animal Science, 2008, Vol 86, Issue 12, p3385
- ISSN
0021-8812
- Publication type
Article
- DOI
10.2527/jas.2008-1051