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- Title
Pharmaco-modulation of splenic inflammatory response by histamine receptors during scorpion envenomation pathogenesis.
- Authors
Adi-Bessalem, Sonia; Lamraoui, Amal; Djebari, Fatima Laraba
- Abstract
The inflammatory response is a key process in the genesis of the pathological events during scorpion envenomation. The activation and release of proinflammatory mediators such as cytokines and vasoactive substances may play an important role in the pathophysiology of scorpion envenomation. The spleen organ is one of the tissues that could be damaged after scorpion envenomation. The mechanism of the induced splenic inflammation by scorpion venom has not yet been elucidated and was little investigated. The present study aimed to evaluate effects of scorpion venom on this lymphoid organ and to investigate the potential involvement of histamine receptors in the induced splenic inflammatory response by Androctonus australis hector (Aah) scorpion venom. Indeed, selective histamine receptor antagonists (cetirizine, cimetidine, betaserc and JNJ-7777120) were administered before the envenomation of mice. The inflammatory response was evaluated by assessing vascular permeability changes and inflammatory cell recruitment including polymorphonuclear and eosinophil cells into this organ. On the other hand, splenic disorders were evaluated by the measurement of some markers of oxidative/nitrosative stress such as nitric oxide, malondialdehyde and reduced glutathione but also by histopahological analysis. The results showed significant alterations of the splenic tissue marked by an increase of vascular permeability and inflammatory cells recruitment, as well as an increased nitric oxide levels and splenic membranes lipids peroxidation concomitant with reduced antioxidant defense. The histamine H1 and H4 receptor antagonists (cetirizine and JNJ7777120, respectively) seem to be more efficient in the prevention of the induced splenic disorders by scorpion venom than the H2 and the H3 receptor inhibitors (cimetidine and betaserc, respectively). A significant reduction of the vascular permeability, the inflammatory cell infiltration and a marked prevention of oxidative/ nitrosative stress markers were observed. Our results suggest the involvement of histamine receptors in the induced splenic inflammatory response by scorpion venom, especially the H1 and the H4 receptors but suggest also the usefulness of their inhibitors in the amelioration of the therapeutic management of scorpion envenomation cases.
- Subjects
INFLAMMATORY mediators; CYTOKINES; SCORPION venom; HISTAMINE receptors; ANDROCTONUS; CETIRIZINE
- Publication
Turkish Journal of Immunology, 2016, Vol 4, p35
- ISSN
1301-109X
- Publication type
Article