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- Title
Aqueous medium enzymatic preparation of l-alpha glycerylphosphorylcholine optimized by response surface methodology.
- Authors
Zhang, Kangyi; Wang, Xingguo; Liu, Yuanfa
- Abstract
The ability of Rhizopus chinensis lipase (Thermo 4S-3) to catalyze the deacylation of l,2-diacyl-sn-glycero-3-phosphocholines (sn-1,2-PC) for l-alpha glycerylphosphorylcholine ( l-α-GPC) preparation was investigated. Response surface methodology (RSM), based on a modified central composite rotatable design, was employed to examine the effects of substrate concentrations, temperature, enzyme loading, and dosage of CaCl on the l-α-GPC yield. RSM analysis indicated good correlation between experimental and predicated values. The optimal condition was confirmed as follows: reaction time, 3.5 h; temperature, 43 °C; enzyme loading, 28.2 U mL; substrate concentration, 51.5 mg mL; and dosage of CaCl, 1.9 mg mL. Under these conditions, the l-α-GPC yield increased by 96.8%, which was close to the amount predicted by the model.
- Subjects
ENZYMATIC analysis; GLYCERYLPHOSPHORYLCHOLINE; MUCORACEAE; RESPONSE surfaces (Statistics); TEMPERATURE effect; MATHEMATICAL models
- Publication
European Food Research & Technology, 2012, Vol 234, Issue 3, p485
- ISSN
1438-2377
- Publication type
Article
- DOI
10.1007/s00217-011-1655-x