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- Title
Manassantin A inhibits cAMP-induced melanin production by down-regulating the gene expressions of MITF and tyrosinase in melanocytes.
- Authors
Lee, Hwa Dong; Lee, Won-Hee; Roh, Eunmiri; Seo, Chang-Seob; Son, Jong-Keun; Lee, Seung Ho; Hwang, Bang Yeon; Jung, Sang-Hun; Han, Sang-Bae; Kim, Youngsoo
- Abstract
Microphthalmia-associated transcription factor (MITF) is inducible in response to cAMP through the cAMP-responsive element-binding protein (CREB) and plays a pivotal role in the melanocyte-specific expression of tyrosinase or tyrosinase-related proteins (TRPs) for melanin biosynthesis. Manassantin A from Saururus chinensis inhibits cAMP-induced melanin production in B16 melanoma cells. Here, we focused on molecular basis of the antimelanogenic activity. Manassantin A consistently inhibited the cAMP elevator 3-isobutyl-1-methylxanthine (IBMX)- or dibutyryl cAMP-induced melanin production in B16 cells or in melan-a melanocytes by down-regulating the expression of tyrosinase or TRP1 gene. Moreover, manassantin A suppressed MITF induction through IBMX-activated CREB pathway, directly inhibiting the Ser-133 phosphorylation of CREB. However, manassantin A did not affect IBMX-increased cAMP levels in these cells but also other cAMP-dependent melanogenic pathways through post-translational modifications of MITF. This putative molecular mechanism of manassantin A in the inhibition of melanin production suggests its pharmacological potential in skin hyperpigmentation.
- Subjects
LETTERS to the editor; PHENOL oxidase; MELANOCYTES
- Publication
Experimental Dermatology, 2011, Vol 20, Issue 9, p761
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/j.1600-0625.2011.01296.x