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- Title
The standard operating procedure of the DOE-JGI Microbial Genome Annotation Pipeline (MGAP v.4).
- Authors
Huntemann, Marcel; Ivanova, Natalia; Mavromatis, Konstantinos; Tripp, H.; Paez-Espino, David; Palaniappan, Krishnaveni; Szeto, Ernest; Pillay, Manoj; Chen, I-Min; Pati, Amrita; Nielsen, Torben; Markowitz, Victor; Kyrpides, Nikos
- Abstract
The DOE-JGI Microbial Genome Annotation Pipeline performs structural and functional annotation of microbial genomes that are further included into the Integrated Microbial Genome comparative analysis system. MGAP is applied to assembled nucleotide sequence datasets that are provided via the IMG submission site. Dataset submission for annotation first requires project and associated metadata description in GOLD. The MGAP sequence data processing consists of feature prediction including identification of protein-coding genes, non-coding RNAs and regulatory RNA features, as well as CRISPR elements. Structural annotation is followed by assignment of protein product names and functions.
- Subjects
MICROBIAL genomes; NON-coding RNA; ANNOTATIONS; METADATA; COMPARATIVE studies; NUCLEOTIDE sequence
- Publication
Standards in Genomic Sciences, 2015, Vol 10, Issue 1, p1
- ISSN
1944-3277
- Publication type
Article
- DOI
10.1186/s40793-015-0077-y