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- Title
Biochemical, Histopathological and Immunohistochemical Evaluation of the Protective and Therapeutic Effects of Thymoquinone against Ischemia and Ischemia/Reperfusion Injury in the Rat Ovary.
- Authors
Mete Ural, Ülkü; Bayoğlu Tekin, Yeşim; Şehitoğlu, İbrahim; Kalkan, Yıldıray; Cumhur Cüre, Medine
- Abstract
<bold>Aim: </bold>To evaluate the antioxidant effects of thymoquinone (TQ) and to investigate the biochemical, histopathological and immunohistochemical changes in experimental rat ovarian torsion.<bold>Methods: </bold>A total of 48 female adult rats were used in this study and randomly divided into 7 groups: (1) sham operation; (2) bilateral 3-hour ovarian ischemia; (3) 3-hour ischemia and 3-hour reperfusion; (4) and (5) rats were administered 20 and 40 mg/kg of TQ, respectively, before 0.5 h of ischemia, and then 3 h of ovarian ischemia was applied; (6) and (7) 3-hour ovarian ischemia was applied; 2.5 h after the induction of ischemia, rats were administered the same doses of TQ; at the end of 3 h of ischemia, a 3-hour reperfusion was applied. Histologic changes under light microscopy, immunoreactivity for anticaspase-3 and serum levels of malondialdehyde, interleukin-6, catalase and glutathione peroxidase were noted and compared between the 7 groups.<bold>Results: </bold>Ischemia and ischemia/reperfusion cause a deterioration of biochemical and histopathological parameters. Administration of TQ seems to reverse these alterations and alleviate the injury. Antioxidant defense mechanisms appear to be enhanced by the administration of TQ.<bold>Conclusion: </bold>TQ at different doses attenuates ovarian ischemia and ischemia/reperfusion injury in rats.
- Subjects
QUINONE derivatives; BIOCHEMISTRY; HISTOPATHOLOGY; TREATMENT of reperfusion injuries; OVARIES; LABORATORY rats; WOUNDS &; injuries; ISCHEMIA prevention; ISCHEMIA treatment; OVARIAN diseases; THERAPEUTICS; REPERFUSION injury; ANIMAL experimentation; BIOLOGICAL models; RATS; BENZOQUINONES; PREVENTION
- Publication
Gynecologic & Obstetric Investigation, 2016, Vol 81, Issue 1, p47
- ISSN
0378-7346
- Publication type
journal article
- DOI
10.1159/000431220