We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Control of the Fluorescence of DNA-templated Silver Nanoclusters by AdenosineTriphosphate and Mercury(II).
- Authors
Chen, Ying ‐ Chieh; Wang, Chia ‐ Wei; Lee, Jennifer D.; Chen, Po ‐ Cheng; Chang, Huan ‐ Tsung
- Abstract
Several DNA templates with the sequence 5'-T n TAACCCCTAACCCCT-3' (n = 0, 15, 30, and 45) were used to prepare DNA template-silver nanoclusters (DNA-Ag NCs). The T n sequence acts as a recognition element for Hg2+, while the rest of the sequence acts as a template for DNA-Ag NCs. At pH 3.0, the fluorescence intensity of DNA-Ag NCs is enhanced by ATP, and the enhanced fluorescence is quenched by Hg2+. The length of polyT shows a slight effect on the sensitivity for the detection of Hg2+ but almost no effect on the optical properties of DNA-Ag NCs. The fluorescence response of DNA-Ag NCs (T15-DNA-Ag NCs) vs. Hg2+ concentration shows two linear ranges over 10-100 and 100-1000 nM, mainly because of the fluorescence quenching due to DNA conformational changes through T-Hg2+-T coordination and the formation of an amalgam with Ag NCs, respectively. The sensitivity of the T15-DNA-Ag NC probe was validated through the analysis of Hg2+ in spiked pond water. Based on the switch-on and switch-off fluorescence properties of T15-DNA-Ag NCs, an IMPLICATION logic gate was fabricated using the concentrations of ATP and Hg2+ as inputs and the fluorescence intensity at 585 nm as output.
- Subjects
FLUORESCENCE; SILVER nanoparticles; ADENOSINE triphosphate; MERCURY; FLUORESCENCE quenching
- Publication
Journal of the Chinese Chemical Society, 2017, Vol 64, Issue 1, p8
- ISSN
0009-4536
- Publication type
Article
- DOI
10.1002/jccs.201600246