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- Title
Y ersinia pseudotuberculosis uses Ail and YadA to circumvent neutrophils by directing Yop translocation during lung infection.
- Authors
Paczosa, Michelle K.; Fisher, Michael L.; Maldonado‐Arocho, Francisco J.; Mecsas, Joan
- Abstract
A Y ersinia pseudotuberculosis ( Yptb ) murine model of lung infection was previously developed using the serotype III IP2666 NdeI strain, which robustly colonized lungs but only sporadically disseminated to the spleen and liver. We demonstrate here that a serotype Ib Yptb strain, IP32953, colonizes the lungs at higher levels and disseminates more efficiently to the spleen and liver compared with IP2666 NdeI. The role of adhesins was investigated during IP32953 lung infection by constructing isogenic Δ ail , Δ inv , Δ psaE and Δ yadA mutants. An IP32953Δ ailΔ yadA mutant initially colonized but failed to persist in the lungs and disseminate to the spleen and liver. Yptb expressing these adhesins selectively bound to and targeted neutrophils for translocation of Yops. This selective targeting was critical for virulence because persistence of the Δ ailΔ yadA mutant was restored following intranasal infection of neutropenic mice. Furthermore, Ail and YadA prevented killing by complement-mediated mechanisms during dissemination to and/or growth in the spleen and liver, but not in the lungs. Combined, these results demonstratethat Ail and YadA are critical, redundant virulence factors during lung infection, because they thwart neutrophils by directing Yop-translocation specifically into these cells.
- Subjects
YERSINIA pseudotuberculosis; LUNG infections; SEROTYPES; BACTERIAL adhesins; NEUTROPHIL immunology; CHROMOSOMAL translocation; MICROBIAL virulence
- Publication
Cellular Microbiology, 2014, Vol 16, Issue 2, p247
- ISSN
1462-5814
- Publication type
Article
- DOI
10.1111/cmi.12219