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- Title
Comparative Gene Expression Analysis of Immune-Related Cytokines in Riemerella anatipestifer-stimulated Philippine Banaba Native Chicken and Native Duck Embryonic Fibroblasts.
- Authors
Fernandez-Colorado, Cherry P.; Desamero, Mark Joseph M.; Salamat, Saubel Ezrael A.; Torno, Gordon Karl Barbour M.; Untalan, Kane Errol M.; Kindipan, Kiariza V.; Fatalla, John-John R.; Linatoc, Ron Carlos R.; Tibar, Jennelyn Joyce D.
- Abstract
Background: Studies on Philippine Banaba native chickens and Philippine Mallard ducks were generally focused on their production and management. However, little is known on their immune responses to pathogens like Riemerella anatipestifer (R. anatipestifer), one of the economically important infectious disease-causing pathogens in Philippine Banaba native chickens and Philippine Mallard ducks. Methods: Chicken and duck embryonic fibroblasts (CEFs and DEFs) isolated from 11-day old healthy embryos were stimulated with heat-inactivated R. anatipestifer for 4, 8 and 24 h. The cells were collected at each time point and subjected to qRT-PCR for gene expression analyses. Results: Comparative gene expression analyses of immune-related cytokines revealed that the ex- pression levels of IL-17A, IL-17F, IL-1ß and IL-6 were significantly upregulated in both CEFs and DEFs stimulated with heat-inactivated R. anatipestifer serotype 7. However, levels were higher in DEFs compared to the corresponding levels in heat-inactivated R. anatipestifer stimulated CEFs. On the other hand, IFN-γ, IL-2, IL-4 and IL-10 expression levels of stimulated DEFs were also upregulated. In CEFs, it is interesting to note that IFN-γ level was significantly downregulated at 24 h while upregulated in DEFs. Conclusion: Results showed that the Philippine Banaba native chickens and Mallard ducks are also susceptible to R. anatipestifer infection.
- Subjects
MALLARD; CHICKENS; GENE expression; FIBROBLASTS; DUCKS
- Publication
Philippine Journal of Veterinary Medicine, 2024, Vol 61, Issue 1, p1
- ISSN
0031-7705
- Publication type
Article