We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
MORPHOLOGICAL AND GROWTH CHARACTERISTICS OF PRIMARY CELL CULTURES DERIVED FROM PHILIPPINE NATIVE BANABA AND RHODE ISLAND RED CHICKEN (Gallus gallus domesticus L.).
- Authors
Pangga, Gladys Maria V.; Collantes, Therese Marie A.
- Abstract
Primary cell cultures of chicken embryo fibroblasts derived from Philippine native Banaba chicken (BNB) and Rhode Island Red (RIR) chicken (Gallus gallus domesticus) were successfully established using the warm trypsin culture method. Cell morphology, viability, confluence, and dynamic growth were observed. The results showed that the extracted cells for both treatments were healthy exhibiting characteristics of fusiform and fibrous structure, reaching 95% confluence at 72 hr. The population doubling time ranged from 60 to 63 hr. Four passages of primary cells of BNB were successfully conducted. The cells in the second passage were preserved with 97.3% cell viability via cell cryogenic technology. The growth curve of the cells projected a typical "S" shape. T-test for two independent means (p<0.05) showed that there were no significant differences in the mean cell concentration and cell viabilities between RIR and BNB cultures. Microscopic analysis of the culture showed that there was no apparent microbial contamination. Cell culture of Banaba chicken fibroblast cells can provide genetic resources for future genomic studies. In addition, the methodology applied in this study proved to be reproducible and can be used as basis for other applicable researches.
- Subjects
CHICKENS; CELL culture; PRIMARY cell culture; CELL morphology; CHICKEN embryos; MICROBIAL contamination
- Publication
Philippine Journal of Veterinary Medicine, 2019, Vol 56, Issue 1, p47
- ISSN
0031-7705
- Publication type
Article