We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Nelumbo nucifera Leaves Prevent NMU-Induced Mammary Tumor through Downregulation of Fatty Acid Synthase, Estrogen Receptor-α and Her2 Expression.
- Authors
Hsu, Li-Sung; Chang, Chun-Hua; Lee, Yi-Ju; Wang, Chau-Jong
- Abstract
Diet polyphenol can reportedly prevent the formation of breast-cancer cells. Nelumbo nucifera leaf extract (NLE) is enriched with polyphenols and has several cellular functions, such as anti-atherosclerosis, anti-inflammation, and antitumor. In this study, we investigated the role of NLE in the prevention of N-methyl-N-nitrosourea (NMU)-induced mammary tumor formation. Cotreatment with NLE significantly reduced the NMU-induced tumor incidence, number, and volume. NLE administration significantly repressed the tumor growth and weight of nude mice upon inoculation with BT-474 cancer cells. Immunohistochemical staining indicated that fatty acid synthetase, estrogen receptor (ER)- α , and phosphorylated ER- α were obviously reduced in the cancer part of BT-474 inoculated nude mice upon administration of 2% NLE. Western blot analysis revealed that NLE and NLPE (polyphenol-rich NLE) repressed ER- α expression and phosphorylation and decreased the phosphorylation of Her-2 without affecting their expression. Overall, NLE and NLPE exhibited more effective antitumor abilities in NMU-induced mammary cancer formation than with tamoxifen and Herceptin.
- Subjects
BREAST tumor prevention; ENZYME metabolism; ANIMAL experimentation; BREAST tumors; CELL lines; CELL receptors; ESTROGEN receptors; GENE expression; IMMUNOHISTOCHEMISTRY; LEAVES; MEDICINAL plants; MICE; ONCOGENES; RATS; RESEARCH funding; T-test (Statistics); WESTERN immunoblotting; PLANT extracts; DATA analysis software; DESCRIPTIVE statistics
- Publication
American Journal of Chinese Medicine, 2019, Vol 47, Issue 8, p1885
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X19500964