We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Real-Time Fluorescence Turn-On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe.
- Authors
Chen, Jian; Jiao, Huping; Li, Wenying; Liao, Dongli; Zhou, Huipeng; Yu, Cong
- Abstract
A tetracationic perylene probe (probe 1) was designed and synthesized. Probe 1 was used for the real-time fluorescence turn-on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe 1 monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe 1 complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe 1 complex, and the recovery of the probe 1 monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.
- Publication
Chemistry - An Asian Journal, 2013, Vol 8, Issue 1, p276
- ISSN
1861-4728
- Publication type
Article
- DOI
10.1002/asia.201200880