We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Mesenchymal stem cells combined with barrier domes enhance vertical bone formation.
- Authors
Zigdon‐Giladi, Hadar; Lewinson, Dina; Bick, Tova; Machtei, Eli E.
- Abstract
Aim To enhance vertical bone formation in a rat calvarium following combination of guided bone regeneration ( GBR) and transplantation of bone marrow derived mesenchymal stem cells (bm MSC). Materials and Methods Gold domes (7 mm radius, 5 mm height) were filled with 5 × 105 bm MSC or osteogenic transformed bm MSC (ot MSC) that were isolated from tibia of inbred rats and mixed with β TCP. Domes filled with β TCP served as control. Rats were sacrificed after 3 months. New bone formation was analysed by histology and histomorphometry. Results In all rats hard tissue filled the space under the dome. In the lower part of the specimens the newly formed mature bone was continuous with the original calvaria, whereas the upper (distal) part of the augmented tissue contained residual scaffold surrounded by connective tissue. Histomorphometric analysis revealed that cell transplantation doubled vertical bone height: (bm MSC 4.13 ± 0.5 mm, ot MSC 4.14 ± 0.3 mm β TCP 2.29 ± 0.22 mm, p ≤ 0.001). Bone area fraction (%) was significantly increased following transplantation of ot MSC (47.2 ± 2.5%) when compared with bm MSC (37.3 ± 3.35%) and with β TCP (31.09 ± 2.7%) ( p ≤ 0.031 versus bm MSC, p ≤ 0.0004 versus control) Conclusion In a rat calvaria model transplantation of both ot MSC and bm MSC, when combined with GBR significantly enhanced bone formation.
- Subjects
ISRAEL; BONES; ACADEMIC medical centers; ALVEOLAR process; ANALYSIS of variance; ANIMAL experimentation; BONE growth; RATS; RESEARCH funding; STATISTICS; STEM cells; DATA analysis; EQUIPMENT &; supplies; ANATOMY
- Publication
Journal of Clinical Periodontology, 2013, Vol 40, Issue 2, p196
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.12044