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- Title
The cross talk between cervical carcinoma cells and vascular endothelial cells mediated by IL-27 restrains angiogenesis.
- Authors
Zhang, Bing; Xie, Feng; Dong, Chun‐Lin; Gu, Chun‐Jie; Cheng, Jiao; Wang, Yuan; Xu, Xi‐Zhong; Pu, Hong; Wu, Yi‐Bo; Qi, Xiao‐Wei; Li, Da‐Jin; Yu, Jin‐Jin; Li, Ming‐Qing
- Abstract
Problem To explore whether cervical carcinoma cell-derived interleukin-27 ( IL-27) modulates the angiogenesis of vascular endothelial cells. Method of study The expression of IL-27 in cervical cancer tissues and cervical cell lines was analyzed by immunohistochemistry, ELISA and flow cytometry. Then, the effects of IL-27 on the proliferation and apoptosis-related molecules and angiogenesis in vitro of human umbilical vein endothelial cells ( HUVECs) were investigated. Finally, in vivo experiment was performed to further confirm the effects of IL-27. Results Compared with cervicitis, the cervical cancer tissues highly expressed IL-27. Both HeLa and CaSki cells secreted IL-27, and HUVECs expressed low levels of IL-27 receptors ( IL-27R). However, the co-culture of cervical cell lines and HUVECs led to a significant elevation of IL-27R on HUVECs. Co-culturing with IL-27-overexpressed HeLa cells downregulated Ki-67 and Bcl-2 and upregulated Fas expression in HUVECs. In addition, overexpression of IL-27 in HeLa cells and CasKi cells secreted less IL-8 and could further restrict angiogenesis compared with control cells in vitro. In the subcutaneous tumorous model of C57/ BL6 mouse, there were decreased vessel density and tumor volume when inoculation with IL-27-overexpressed TC-1 cells. Conclusion This study indicates that IL-27 secreted by cervical carcinoma cells restricts the angiogenesis in a paracrine manner in the pathogenesis of cervical cancer.
- Subjects
CERVICAL cancer; CANCER cells; VASCULAR endothelial cells; NEOVASCULARIZATION; IMMUNOHISTOCHEMISTRY
- Publication
American Journal of Reproductive Immunology, 2017, Vol 78, Issue 4, pn/a
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12706