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- Title
Frequency of azole resistance in clinical and environmental strains of Aspergillus fumigatus in Turkey: a multicentre study.
- Authors
Ener, Beyza; Ergin, Çağrı; Gülmez, Dolunay; Ağca, Harun; Tikveşli, Melek; Aksoy, Seçil Ak; Otkun, Müşerref; Siğ, Ali Korhan; Öğünç, Dilara; Özhak, Betil; Topaç, Tuncay; Özdemir, Aslı; Metin, Dilek Yeşim; Polat, Süleyha Hilmioğlu; Öz, Yasemin; Koç, Nedret; Atalay, Mustafa Altay; Erturan, Zayre; Birinci, Asuman; Çerikçioğlu, Nilgün
- Abstract
<bold>Objectives: </bold>Aspergillus fumigatus causes several diseases in humans and azole resistance in A. fumigatus strains is an important issue. The aim of this multicentre epidemiological study was to investigate the prevalence of azole resistance in clinical and environmental A. fumigatus isolates in Turkey.<bold>Methods: </bold>Twenty-one centres participated in this study from 1 May 2018 to 1 October 2019. One participant from each centre was asked to collect environmental and clinical A. fumigatus isolates. Azole resistance was screened for using EUCAST agar screening methodology (EUCAST E.DEF 10.1) and was confirmed by the EUCAST E.DEF 9.3 reference microdilution method. Isolates with a phenotypic resistance pattern were sequenced for the cyp51A gene and microsatellite genotyping was used to determine the genetic relationships between the resistant strains.<bold>Results: </bold>In total, resistance was found in 1.3% of the strains that were isolated from environmental samples and 3.3% of the strains that were isolated from clinical samples. Mutations in the cyp51A gene were detected in 9 (47.4%) of the 19 azole-resistant isolates, all of which were found to be TR34/L98H mutations. Microsatellite genotyping clearly differentiated the strains with the TR34/L98H mutation in the cyp51A gene from the strains with no mutation in this gene.<bold>Conclusions: </bold>The rate of observed azole resistance of A. fumigatus isolates was low in this study, but the fact that more than half of the examined strains had the wild-type cyp51A gene supports the idea that other mechanisms of resistance are gradually increasing.
- Subjects
TURKEY; ANTIFUNGAL agents; PROTEINS; RESEARCH; HETEROCYCLIC compounds; RESEARCH methodology; EVALUATION research; ASPERGILLOSIS; COMPARATIVE studies; RESEARCH funding; DRUG resistance in microorganisms; ASPERGILLUS; MICROBIAL sensitivity tests; PHARMACODYNAMICS
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2022, Vol 77, Issue 7, p1894
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dkac125