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- Title
Preliminary experiments on use of zebrafish as a laboratory model for Giardia duodenalis infection.
- Authors
Tysnes, Kristoffer; Jørgensen, Anders; Poppe, Trygve; Midtlyng, Paul; Robertson, Lucy
- Abstract
Although Giardia duodenalis is considered a parasite of mammals, different genotypes have been identified as infecting several species of freshwater and marine fish in Australia. Establishment of G. duodenalis infection in common laboratory zebrafish ( Danio rerio), could provide an excellent tool for a range of studies on Giardia. We conducted preliminary experiments to investigate this possibility. Zebrafish were inoculated with viable G. duodenalis cysts from two different Assemblages (A and D) using a modified oro-gastric tube. Direct microscopy and immunofluorescent antibody test were used to check for Giardia cysts/trophozoites in the intestine, and histology was performed on intestinal mucosa to evaluate possible pathological changes. Giardia cysts were successfully deposited in the zebrafish alimentary tract using a modified oro-gastric tube, and were maintained in the fish gut for at least 8 days. Although a single trophozoite was observed in one fish three days post-exposure, we were unable to demonstrate established, propagative infection under the conditions tested.
- Subjects
AUSTRALIA; GIARDIA; HEXAMITIDAE; MARINE fishes; FRESHWATER fishes; ZEBRA danio
- Publication
Acta Parasitologica, 2012, Vol 57, Issue 1, p1
- ISSN
1230-2821
- Publication type
Article
- DOI
10.2478/s11686-012-0001-1