We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
n-3 Fatty Acids Preserve Insulin Sensitivity In Vivo in a Peroxisome Proliferator-Activated Receptor-α-Dependent Manner.
- Authors
Neschen, Susanne; Morino, Katsutaro; Dong, Jianying; Wang-Fischer, Yanlin; Cline, Gary W.; Romanelli, Anthony J.; Rossbacher, Jörg C.; Moore, Irene K.; Regittnig, Werner; Munoz, David S.; Kim, Jung H.; Shulman, Gerald I.
- Abstract
Recent studies have suggested that n-3 fatty acids, abundant in fish oil, protect against high-fat diet-induced insulin resistance through peroxisome proliferator-activated receptor (PPAR)-α activation and a subsequent decrease in intracellular lipid abundance. To directly test this hypothesis, we fed PPAR-α null and wild-type mice for 2 weeks with isocaloric high-fat diets containing 27% fat from either safflower oil or safflower oil with an 8% fish oil replacement (fish oil diet). In both genotypes the safflower oil diet blunted insulin-mediated suppression of hepatic glucose production (P < 0.02 vs. genotype control) and PEPCK gene expression. Feeding wild-type mice a fish oil diet restored hepatic insulin sensitivity (hepatic glucose production [HGP], P < 0.002 vs. wild-type mice fed safflower oil), whereas in contrast, in PPAR-α null mice failed to counteract hepatic insulin resistance (HGP, P = NS vs. PPAR-α null safflower off-fed mice). In PPAR-α null mice fed the fish oil diet, safflower oil plus fish oil, hepatic insulin resistance was dissociated from increases in hepatic triacylglycerol and acyl-CoA but accompanied by a more than threefold increase in hepatic diacylglycerol concentration (P < 0.0001 vs. genotype control). These data support the hypothesis that n-3 fatty acids protect from high-fat diet-induced hepatic insulin resistance in a PPAR-α- and diacylglycerol-dependent manner. Diabetes 56:1034-1041, 2007
- Subjects
OMEGA-3 fatty acids; INSULIN resistance; PEROXISOMES; LABORATORY mice; LIPIDS
- Publication
Diabetes, 2007, Vol 56, Issue 4, p1034
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/db06-1206