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- Title
P1G10, the Proteolytic Fraction from Vasconcellea cundinamarcensis, Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation.
- Authors
Freitas, Kátia M.; Silva, Ana C. Araújo e; Veloso, Emerson S.; Ferreira, Ênio; Barcelos, Lucíola S.; Caliari, Marcelo V.; Salas, Carlos E.; Lopes, Miriam T. P.
- Abstract
Background: P1G10 is a cysteine proteolytic fraction from Vasconcellea cundinamarcensis latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was suggested an antioxidant role. Methods: We evaluated here the effect of topical treatment with P1G10, in mice lesions induced by UVB. Results: After single exposure to 2.4 J cm−2 UVB, P1G10 reduced erythema, increased cellularity of hypodermis, enhanced MPO activity and IL1β, and inhibited COX2 levels. These results point to an anti-inflammatory effect by P1G10. This fraction displayed antioxidant activity by reversing the depletion of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reducing the catalase activity increased by UVB. These changes may be related to a reduction in MDA observed in groups treated with P1G10. P1G10 also inhibited MMP-9, caspase-3 and pkat while increasing p53 levels.
- Subjects
ULTRAVIOLET radiation; SUPEROXIDE dismutase; FRACTIONS; ULTRAFILTRATION; TISSUES; PALMITOYLATION
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 18, p4373
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20184373