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- Title
Alteration of Dynein Function Affects ɑ-Synuclein Degradation via the Autophagosome-Lysosome Pathway.
- Authors
Da Li; Ji-Jun Shi; Cheng-Jie Mao; Sha Liu; Jian-Da Wang; Jing Chen; Fen Wang; Ya-Ping Yang; Wei-Dong Hu; Li-Fang Hu; Chun-Feng Liu
- Abstract
Growing evidence suggests that dynein dysfunction may be implicated in the pathogenesis of neurodegeneration. It plays a central role in aggresome formation, the delivery of autophagosome to lysosome for fusion and degradation, which is a pro-survival mechanism essential for the bulk degradation of misfolded proteins and damaged organells. Previous studies reported that dynein dysfuntion was associated with aberrant aggregation of ɑ-synuclein, which is a major component of inclusion bodies in Parkinson's disease (PD). However, it remains unclear what roles dynein plays in ɑ-synuclein degradation. Our study demonstrated a decrease of dynein expression in neurotoxin-induced PD models in vitro and in vivo, accompanied by an increase of ɑ-synuclein protein level. Dynein down-regulation induced by siRNA resulted in a prolonged half-life of ɑ-synuclein and its over-accumulation in A53T overexpressing PC12 cells. Dynein knockdown also prompted the increase of microtubule-associated protein 1 light chain 3 (LC3-II) and sequestosome 1 (SQSTM1, p62) expression, and the accumulation of autophagic vacuoles. Moreover, dynein suppression impaired the autophagosome fusion with lysosome. In summary, our findings indicate that dynein is critical for the clearance of aberrant ɑ-synuclein via autophagosome-lysosome pathway.
- Subjects
DYNEIN; SYNUCLEINS; AUTOPHAGY; LYSOSOMES; BRAIN diseases
- Publication
International Journal of Molecular Sciences, 2013, Vol 14, Issue 12, p24242
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms141224242