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- Title
A Determination of Potential &agr;-Glucosidase Inhibitors from Azuki Beans (Vigna angularis).
- Authors
Yang Yao; Xuzhen Cheng; Lixia Wang; Suhua Wang; Guixing Ren
- Abstract
A 70% ethanol extract from azuki beans (Vigna angularis) was extracted further with CH2Cl2, EtOAc and n-BuOH to afford four fractions: CH2Cl2-soluble, EtOAc-soluble, n-BuOH-soluble and residual extract fractions. The EtOAc-soluble fractions showed the highest a-glucosidase inhibitory activity. Two pure flavonoid compounds, vitexin and isovitexin, were isolated (using the enzyme assay-guide fractionation method) from the EtOAc-soluble fractions. We further evaluated the interaction between the flavonoid compounds and a-glucosidase by fluorescence spectroscopy. Vitexin and isovitexin showed high inhibitory activities, with IC50 values of 0.4 mg·mL-1 and 4.8 mg·mL-1, respectively. This is the first study of the active compositions of azuki beans against &agr;-glucosidase.
- Subjects
GLUCOSIDASE inhibitors; AZUKI; ETHANOL; FLAVONOIDS; GLUCOSIDASES; FLUORESCENCE spectroscopy
- Publication
International Journal of Molecular Sciences, 2011, Vol 12, Issue 10, p6445
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms12106445