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- Title
Inner limiting membrane as membranous support in RPE sheet-transplantation.
- Authors
Julia Beutel; Lena Greulich; Matthias Lüke; Focke Ziemssen; Peter Szurman; Karl-Ulrich Bartz-Schmidt; Salvatore Grisanti
- Abstract
Abstract Purpose  To investigate the inner limiting membrane as a scaffold for retinal pigment epithelium cells (RPE). Methods  Human donor eyes (nâ=â10) from the Eye Bank T�bingen were used to collect the inner limiting membrane (ILM). These human donor eyes and additional porcine eyes (nâ=â11) served to isolate RPE cells. A human RPE cell line (ARPE-19) was used as control. RPE cells were cultured on ILM for 3 and 7 days. Phase-contrast photographs of the cells in culture were obtained. Morphology and ultrastructural changes were evaluated by light and transmission electron microscopy. Results  Porcine RPE cells adhere and proliferate when seeded on human ILM. The cells maintained their cuboidal morphology, were polarized, disclosed microvilli on the apical surface, formed intercellular junctions and did not dedifferentiate. Human RPE cells obtained from cadaver eyes barely adhered to the ILM and did not form an intact monolayer. ARPE-19 cells formed a dense colony and maintained epithelial features. Conclusion  The ILM is an ideal matrix to establish an intact RPE monolayer and has the potential to be used as sheet for subretinal transplantation.
- Subjects
RETINOIDS; CELL culture; EPITHELIUM; CELL lines
- Publication
Graefe's Archive of Clinical & Experimental Ophthalmology, 2007, Vol 245, Issue 10, p1469
- ISSN
0721-832X
- Publication type
Article
- DOI
10.1007/s00417-007-0566-9