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- Title
Upregulation of Enzymes Metabolizing 5-Fluorouracil in Colorectal Cancer.
- Authors
Matsusaka, Satoshi; Yamasaki, Hajime; Fukushima, Masakazu; Wakabayashi, Ichiro
- Abstract
Background: The purpose of this study was to compare the activities of various enzymes, participating in the metabolism of 5-fluorouracil, between colorectal cancer and nontumor tissues and to investigate the association of the enzyme activities with clinicopathological backgrounds. Methods: Activities of seven enzymes involved in nucleic acid metabolism – orotate phosphoribosyltransferase (OPRT), ribonucleotide reductase (RNR), thymidylate synthase, dihydropyrimidine dehydrogenase, thymidine phosphorylase, uridine phosphorylase and thymidine kinase (TK) – were measured in tumor and nontumor tissues from 28 patients who were operated on for colorectal cancers. Results: OPRT, thymidylate synthase, RNR, thymidine phosphorylase, uridine phosphorylase and thymidine kinase activities were significantly higher in tumor areas than in nontumor areas. OPRT showed the highest T/N ratio (the ratio of each enzyme activity in tumor areas to that in nontumor areas). The T/N ratio of RNR activity showed a tendency to be associated with lymph node metastasis and Dukes classification. Conclusion: The results suggest that OPRT is a main enzyme participating in the phosphorylation of 5-fluorouracil and has an important role in tumor growth. The T/N ratio of RNR may be predictive of tumor progression. Copyright © 2007 S. Karger AG, Basel
- Subjects
ENZYMES; FLUOROURACIL; COLON cancer; CANCER; ANTINEOPLASTIC agents
- Publication
Chemotherapy (0009-3157), 2007, Vol 53, Issue 1, p36
- ISSN
0009-3157
- Publication type
Article
- DOI
10.1159/000098249