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- Title
Magnitude of CD8<sup>+</sup> T-cell responses against hepatitis C virus and severity of hepatitis do not necessarily determine outcomes in acute hepatitis C virus infection.
- Authors
Doi, Hiroyoshi; Hiroishi, Kazumasa; Shimazaki, Tomoe; Eguchi, Junichi; Baba, Toshiyuki; Ito, Takayoshi; Matsumura, Takuya; Nozawa, Hisako; Morikawa, Kenichi; Ishii, Shigeaki; Hiraide, Ayako; Sakaki, Masashi; Imawari, Michio
- Abstract
Aim: We investigated the relationship between the magnitude of comprehensive hepatitis C virus (HCV)-specific CD8+ T-cell responses and the clinical course of acute HCV infection. Methods: Six consecutive patients with acute HCV infection were studied. Analysis of HCV-specific CD8+ T-cell responses was performed using an interferon-γ-based enzyme-linked immunospot assay using peripheral CD8+ T-cells, monocytes and 297 20-mer synthetic peptides overlapping by 10 residues and spanning the entire HCV sequence of genotype 1b. Results: Five patients presented detectable HCV-specific CD8+ T-cell responses against a single and different peptide, whereas 1 patient showed responses against three different peptides. Neither the magnitude of HCV-specific CD8+ T-cell responses nor the severity of hepatitis predicts the outcome of acute hepatitis. The maximum number of HCV-specific CD8+ T-cells correlated with maximum serum alanine aminotransferase level during the course ( r = 0.841, P = 0.036). Conclusions: HCV-specific CD8+ T-cell responses were detectable in all 6 patients with acute HCV infection, and 6 novel HCV-specific CTL epitopes were identified. Acute HCV infection can resolve with detectable HCV-specific CD8+ T-cell responses, but without development of antibody against HCV.
- Subjects
T cells; LYMPHOCYTES; TH1 cells; HEPATITIS C virus; FLAVIVIRUSES
- Publication
Hepatology Research, 2009, Vol 39, Issue 3, p256
- ISSN
1386-6346
- Publication type
Article
- DOI
10.1111/j.1872-034X.2008.00459.x