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- Title
Isolation of Primary Mice Splenocytes For In Vitro Research.
- Authors
Ebrahim, Fawzi O.; Abolayha, Abdul M.; Elzagheid, Adam A.; Abosrer, Fadella
- Abstract
Animal cell culture is of great importance in research. We isolated mouse splenocytes from Swiss albino mice by using a mechanical method and cultured them in medium with fetal bovine serum, 1% penicillin-streptomycin and 1% amphotericin as antibiotic and an antifungal, but without mitogen. The cells were confluent after 72 h, and their viability, measured by trypan blue assay, was 95%. The splenocytes were passaged four times and no fungal or bacterial contamination was observed during carrying out the experiment.
- Subjects
ANIMAL culture; BACTERIAL contamination; TRYPAN blue; MICE; CELL culture; ECHINOCANDINS
- Publication
Alexandria Journal of Veterinary Sciences, 2020, Vol 64, Issue 2, p9
- ISSN
1110-2047
- Publication type
Article
- DOI
10.5455/ajvs.82519