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- Title
Prokaryotic Expression and Preparation of Polyclonal Antibody of HtrA Protein from Escherichia coli Causing Pneumonia in Fox.
- Authors
Zhang Zhiqiang; Wu Tonglei; Wu Qingmin; Yan Xijun; Bai Xue; Zhang Yanling; He Hongxuan; Zhang Hongxue; Liu Changhao; Shi Qiumei
- Abstract
To investigate the roles of HtrA protein in bacterial adaption to stresses, htra gene was cloned from an Escherichia coli strain isolated from silver fox, double digested, and ligated into the prokaryotic expression vector pET32a. Then, the recombinant plasmid pET32a-htra was transformed into E. coli BL21 competent cells. The over-expression of His-HtrA protein was induced by the addition of IPTG at a final concentration of 1 mmol/L, and verified by SDA-PAGE and Western blot. In addition, the protein was purified with Ni-NTA agarose, and used to immunize mice,and the polyclonal antibody specifically bound to E. coli HtrA protein. The results will provide a theoretical basis for studying the roles of HtrA protein and developing vaccines for fur-bearing animals against E. coli.
- Subjects
ESCHERICHIA coli proteins; PROTEIN expression; PROKARYOTES; PNEUMONIA; PNEUMONIA in animals; FOXES; IMMUNOLOGY
- Publication
Animal Husbandry & Feed Science, 2018, Vol 10, Issue 1, p58
- ISSN
1943-9911
- Publication type
Article
- DOI
10.19578/j.cnki.ahfs.2018.01.012