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- Title
Use of Light-Emitting Diode Fluorescence Microscopy to Detect Acid-Fast Bacilli in Sputum as Proficient Alternative Tool in the Diagnosis of Pulmonary Tuberculosis in Countries with Limited Resource Settings.
- Authors
Coulibaly, Gagni; Togo, Antièmé Combo Georges; Somboro, Anou Moise; Kone, Mahamadou; Traore, Fah Gaoussou; Diallo, Fatimata; Degoga, Boureima; Somboro, Amadou; Dramé, Hawa M'baye; Sanogo, Moumine; Kodio, Ousmane; Baya, Bocar; Tolofoudie, Mohamed; Maiga, Aminata; Maiga, Mamoudou; Saliba-Shaw, Katy; Diallo, Souleymane; Doumbia, Seydou; Maiga, Ibrahim Izetiégouma; Samaké, Fassé
- Abstract
Background: Despite recent advances in the development of more sensitive technologies for the diagnosis of tuberculosis (TB), in resource-limited settings, the diagnosis continues to rely on sputum smear microscopy. This is because smear microscopy is simple, cost-efficient and the most accessible tool for the diagnosis of TB. Our study evaluated the performance of light-emitting diode fluorescence microscopy (LED-FM) using auramine/rhodamine (auramine) and the fluorescein di-acetate (FDA) vital stain in the diagnostic of pulmonary TB in Bamako, Mali. Methods: Sputum smear microscopy was conducted using the FDA and auramine/rhodamine staining procedures on fresh samples using LED-FM to evaluate the Mycobacterium TB (MTB) metabolic activity and to predict contagiousness. Mycobacterial culture assay was utilized as a gold standard method. Results: Out of 1401 TB suspected patients, 1354 (96.65%) were retrieved from database, which were MTB complex culture positive, and 47 (3.40%) were culture negative (no mycobacterial growth observed). Out of the 1354 included patients, 1343 (95.86%), were acid-fast bacillus (AFB) positive after direct FDA staining, 1352 (96.50%) AFB positive after direct Auramine, and 1354 (96.65%) AFB positive with indirect auramine after digestion and centrifugation. Overall, the FDA staining method has a sensitivity of 98.82%, while the sensitivity of Auramine with direct observation was 99.48%, and 99.56% with the indirect examination. Conclusion: This study showed that, using fresh sputum both auramine/rhodamine and FDA are highly sensitive methods in diagnosing pulmonary TB and could be easily used in countries with limited resource settings.
- Publication
International Journal of Mycobacteriology, 2023, Vol 12, Issue 2, p144
- ISSN
2212-5531
- Publication type
Article
- DOI
10.4103/ijmy.ijmy_13_23