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- Title
Comparisons among the diagnostic methods used for the detection of extra-pulmonary tuberculosis in Bangladesh
- Authors
Munshi, Saurab Kishore; Rahman, Farjana; Mostofa Kamal, S.M.; Noor, Rashed
- Abstract
Abstract: The present study was an attempt to establish a suitable method for the effective diagnosis of extra-pulmonary tuberculosis in Bangladesh. In this regard, detection of Mycobacterium tuberculosis from 390 different extra-pulmonary specimens was performed by Bright-Field microscopy, light-emitting diode fluorescence microscopy and Lowenstein–Jensen culture methods, followed by an extensive comparison among these methods. M. tuberculosis was detected in 53 cases through the conventional Lowenstein–Jensen culture method; 49 cases were detected under Bright-Field microscope, whereas the light-emitting diode fluorescence microscopy detected 64 cases. Out of 53 culture-positive isolates, 12 were found to be multi-drug resistant. Light-emitting diode fluorescence microscopy was found to be more sensitive and effective than both the Bright-Field microscopy and the Lowenstein–Jensen culture methods. Incidentally, light-emitting diode fluorescence microscopy appeared imperative to detecting the multi-drug resistant tuberculosis.
- Subjects
BANGLADESH; TUBERCULOSIS diagnosis; COMPARATIVE studies; MYCOBACTERIUM tuberculosis; FLUORESCENCE microscopy; MULTIDRUG resistance
- Publication
International Journal of Mycobacteriology, 2012, Vol 1, Issue 4, p190
- ISSN
2212-5531
- Publication type
Article
- DOI
10.1016/j.ijmyco.2012.10.004