We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A reference genome for Bluegill (Centrarchidae: Lepomis macrochirus).
- Authors
Ludt, William B.; Corbett, Eamon C.; Kattawar III, Jerry; Chakrabarty, Prosanta; Faircloth, Brant C.
- Abstract
North American sunfishes (Family Centrarchidae) are among the most popular sportfish throughout the United States and Canada. Despite the popularity of sunfishes, their ecological importance, and their extensive stocking and aquacultural history, few molecular studies have examined the evolutionary relationships and species boundaries among members of this group, many of which are known to hybridize. Here, we describe a chromosome-scale genome assembly representing Bluegill (Lepomis macrochirus), one of the most widespread centrarchid species. By combining long-read, Oxford Nanopore sequencing data with short-insert, whole-genome and HiC sequence reads, we produced an assembly (Lm_LA_1.1) having a total length of 889 Mb including 1,841 scaffolds and having a scaffold N50 of 36 Mb, L50 of 12, N90 of 29 Mb, and L90 of 22. We detected 99% (eukaryota_odb10) and 98% (actinopterygii_odb10) universal single-copy orthologs (BUSCOs), and ab initio gene prediction performed using this new assembly identified a set of 17,233 genes that were supported by external (OrthoDB v10) data. This new assembly provides an important addition to the growing set of assemblies already available for spinyrayed fishes (Acanthomorpha), and it will serve as a resource for future studies that focus on the complex evolutionary history of centrarchids.
- Subjects
CANADA; BLUEGILL; ACTINOPTERYGII; SEBASTES marinus; SPECIES; EUKARYOTES
- Publication
G3: Genes | Genomes | Genetics, 2023, Vol 13, Issue 3, p1
- ISSN
2160-1836
- Publication type
Article
- DOI
10.1093/g3journal/jkad019