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- Title
E3 ligases MAC3A and MAC3B ubiquitinate UBIQUITIN-SPECIFIC PROTEASE14 to regulate organ size in Arabidopsis.
- Authors
Guo, Xiaopeng; Zhang, Xin; Jiang, Shan; Qiao, Xin; Meng, Bolun; Wang, Xiaohang; Wang, Yanan; Yang, Kaihuan; Zhang, Yilan; Li, Na; Chen, Tianyan; Kang, Yiyang; Yao, Mengyi; Zhang, Xuan; Wang, Xinru; Zhang, Erling; Li, Junhua; Yan, Dawei; Hu, Zhubing; Botella, José Ramón
- Abstract
The molecular mechanisms controlling organ size during plant development ultimately influence crop yield. However, a deep understanding of these mechanisms is still lacking. UBIQUITIN-SPECIFIC PROTEASE14 (UBP14), encoded by DA3, is an essential factor determining organ size in Arabidopsis (Arabidopsis thaliana). Here, we identified two suppressors of the da3-1 mutant phenotype, namely SUPPRESSOR OF da3-1 1 and 2 (SUD1 and SUD2), which encode the E3 ligases MOS4-ASSOCIATED COMPLEX 3A (MAC3A) and MAC3B, respectively. The mac3a-1 and mac3b-1 mutations partially suppressed the high ploidy level and organ size phenotypes observed in the da3-1 mutant. Biochemical analysis showed that MAC3A and MAC3B physically interacted with and ubiquitinated UBP14/DA3 to modulate its stability. We previously reported that UBP14/DA3 acts upstream of the B-type cyclin-dependent kinase CDKB1;1 and maintains its stability to inhibit endoreduplication and cell growth. In this work, MAC3A and MAC3B were found to promote the degradation of CDKB1;1 by ubiquitinating UBP14/DA3. Genetic analysis suggests that MAC3A and MAC3B act in a common pathway with UBP14/DA3 to control endoreduplication and organ size. Thus, our findings define a regulatory module, MAC3A/MAC3B-UBP14-CDKB1;1, that plays a critical role in determining organ size and endoreduplication in Arabidopsis. Analysis of 2 suppressors uncovers the molecular and genetic mechanisms by which E3 ligases MAC3A and MAC3B affect the stability of UBIQUITIN-SPECIFIC PROTEASE14 to control plant organ size.
- Publication
Plant Physiology, 2024, Vol 194, Issue 2, p684
- ISSN
0032-0889
- Publication type
Article
- DOI
10.1093/plphys/kiad559