We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside α-Hemolysin.
- Authors
Li, Ting; Liu, Lei; Li, Yuru; Xie, Jiani; Wu, Hai-Chen
- Abstract
Nanopore emerged as a powerful single-molecule technique over the past two decades, and has shown applications in the stochastic sensing and biophysical studies of individual molecules. Here, we report a versatile strategy for nanopore sensing by employing the combination of aptamers and host-guest interactions. An aptamer is first hybridized with a DNA probe which is modified with a ferrocene⊂cucurbit[7]uril complex. The presence of analytes causes the aptamer-probe duplex to unwind and release the DNA probe which can quantitatively produce signature current events when translocated through an α-hemolysin nanopore. The integrated use of magnetic beads can further lower the detection limit by approximately two to three orders of magnitude. Because aptamers have shown robust binding affinities with a wide variety of target molecules, our proposed strategy should be universally applicable for sensing different types of analytes with nanopore sensors.
- Subjects
NANOPORES; NANOSTRUCTURED materials; HEMOLYSIS &; hemolysins; APTAMERS; SYNTHETIC antibodies; FERROCENE; ORGANOIRON compounds
- Publication
Angewandte Chemie International Edition, 2015, Vol 54, Issue 26, p7568
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201502047