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- Title
Use of 2-DE and MALDI-TOF-MS to screen differentially expressed serum proteins in patients with oral lichen planus before and after hydroxychloroquine treatment.
- Authors
JIANG Hong-liu; WANG Wen-mei; DUAN Ning; JIANG Wen-hui; WANG Xiang
- Abstract
PURPOSE: To establish 2-dimensional gel eleetrophoresis images and compare the differences of serum proteins of oral lichen planus patients before and after hydroxychloroquine therapy. METHODS: The serum of oral lichen planus patients before and after hydroxyehloroquine therapy were collected, and total protein were extracted. Differential proteome profiles were established and analysed by means of 2-DE and MALDI-TOF-MS. The types and functions of protein were analyzed. SAS 9.12 software package was used for statistical analysis. RESULTS: Six proteins were well characterized including plasminogen precursor, Apo A-IV preeursor,C4A/C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein. The differences were statistically significant. CONCLUSIONS: Plasminogen preeursor,Apo A-IV precursor,C4A!C4B complement, C2 precursor, Vitamin D binding protein and hypothetical protein are differentially expressed in oral lichen planus patients before and after hydroxyehloroquine therapy, but the results need to be validated by other biochemical technologies. Supported by National Natural Science Foundation of China (81070839), Nanjing Talents Project (200603005), Nanjing Medical Science and Technology Development Foundation (ZKX 1030) and Jiangsu Provincial Outstanding Medical Academic Leader Program(LJ201110).
- Subjects
GEL electrophoresis; MATRIX-assisted laser desorption-ionization; MASS spectrometry; GENE expression; BLOOD serum analysis; ORAL lichen planus; CHLOROQUINE; ANTIMALARIALS; THERAPEUTICS
- Publication
Shanghai Journal of Stomatology, 2013, Vol 22, Issue 2, p184
- ISSN
1006-7248
- Publication type
Article