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- Title
Impaired Ultraviolet-B-Induced Cytokine Induction in Xeroderma Pigmentosum Fibroblasts.
- Authors
Suzuki, Hirotake; Kalair, Waseem; Shivji, Gulner M; Wang, Binghe; Toto, Paola; Amerio, Paolo; Kraemer, Kenneth H; Sauder, Daniel N
- Abstract
Xeroderma pigmentosum is a rare, autosomal recessive disease in which patients develop excessive solar damage at an early age and have a 1000-fold increased risk of developing cutaneous neoplasms. Xeroderma pigmentosum can be classified into seven complementation groups (A-G) with defects in different DNA nucleotide excision repair genes. Xeroderma pigmentosum patients also have impaired immune function including reduced natural killer cell activity and impaired induction of interferon-γ. We hypothesized that altered cytokine induction may contribute to the immune defect in xeroderma pigmentosum patients. We examined cytokine mRNA expression after ultraviolet B irradiation using reverse transcriptase polymerase chain reaction in fibroblasts derived from five xeroderma pigmentosum patients in complementation groups A, C, and D and in complemented XP-A and XP-D cells. Cytokines interleukin-1β and interleukin-6 displayed impaired ultraviolet B induction whereas interleukin-8 had normal induction in the xeroderma pigmentosum fibroblasts. Stable complementation of XP-A and XP-D cell lines increased ultraviolet-B-induced interleukin-1β and interleukin-6 expression. These results demonstrate a deficient response of xeroderma pigmentosum fibroblasts to ultraviolet B in terms of cytokine interleukin-1β and interleukin-6 induction but normal interleukin-8 induction and exhibit a role for DNA repair in cytokine induction.
- Subjects
CYTOKINES; XERODERMA pigmentosum; POLYMERASE chain reaction; FIBROBLASTS
- Publication
Journal of Investigative Dermatology, 2001, Vol 117, Issue 5, p1151
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1046/j.0022-202x.2001.01525.x