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- Title
Estradiol acutely inhibits whole body lipid oxidation and attenuates lipolysis in subcutaneous adipose tissue: a randomized, placebo-controlled study in postmenopausal women.
- Authors
Gormsen, Lars Christian; Høst, Christian; Hjerrild, Britta Eilersen; Pedersen, Steen Bønløkke; Nielsen, Søren; Christiansen, Jens Sandahl; Gravholt, Claus Højbjerg
- Abstract
Context: Estradiol (E2) promotes and maintains the female phenotype characterized by subcutaneous fat accumulation. There is evidence to suggest that this effect is due to increased anti-lipolytic α2Aadrenergic receptors, but whether this requires long-term exposure to E2 or is an immediate effect is not clear. Objective: To study acute effects of a single dose (4 mg) of 17β-E2 on regional and systemic lipolysis. Methods: Sixteen postmenopausal women (age, 59±5 years; weight, 67±10 kg; and BMI, 24.8±2.9) were studied in a crossover design: i) placebo and ii) 4 mg E2. Basal and adrenaline-stimulated regional lipolysis was assessed by microdialysis and substrate oxidation rates by indirect calorimetry. Tissue biopsies were obtained to assess lipoprotein lipase activity and mRNA expression of adrenergic, estrogen, cytokine, and vascular reactivity receptors. Results: Acute E2 stimulation significantly attenuated catecholamine-stimulated lipolysis in femoral subcutaneous adipose tissue (interstitial glycerol concentration (micromole/liter) ANOVA time vs treatment interaction, P=0.01) and lipolysis in general in abdominal adipose tissue (ANOVA treatment alone, P<0.05). E2 also reduced basal lipid oxidation ((mg/kg per min) placebo, 0.58±0.06 vs E2, 0.45±0.03; P=0.03) and induced a significantly higher expression of anti-lipolytic α2A-adrenergic receptor mRNA (P=0.02) in skeletal muscle tissue as well as an upregulation of eNOS (NOS3) mRNA (P=0.02). Conclusion: E2 acutely attenuates the lipolytic response to catecholamines in subcutaneous adipose tissue, shifts muscular adrenergic receptor mRNA toward anti-lipolytic α2A-receptors, decreases whole body lipid oxidation, and enhances expression of markers of vascular reactivity.
- Subjects
LIPID peroxidation (Biology); OSTEOPOROSIS in women; ESTRADIOL; ADIPOSE tissues; RANDOMIZED controlled trials; LIPOLYSIS; HUMAN phenotype
- Publication
European Journal of Endocrinology, 2012, Vol 167, Issue 4, p543
- ISSN
0804-4643
- Publication type
Article
- DOI
10.1530/EJE-12-0422