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- Title
Coregulation of HIV-1 dependency factors in individuals heterozygous to the CCR5-delta32 deletion.
- Authors
Hütter, Gero; Blüthgen, Christian; Neumann, Martin; Reinwald, Mark; Nowak, Daniel; Klüter, Harald
- Abstract
Background CCR5-delta32 heterozygous individuals are susceptible to HIV-1. However, it is not clear if there is a relevant protective effect against transmission and a beneficial effect in terms of HIV progression which cannot be attributed to CCR5 surface density alone. Therefore we investigated HIV-1 dependency factors (HDF) which might be differently regulated in CCR5 wild type (WT) and CCR5-delta32 heterozygous individuals. Methods We examined CD34+ hematopoietic progenitor cells derived from bone marrow samples from 19 healthy volunteers, 12 individuals with CCR5 WT and 7 with heterozygous CCR5- delta32 deletion. Samples were analyzed using a global gene expression oligonucleotide microarray (HG-U133plus 2.0, Affymetrix Inc.) Results A total of 205 genes were found with altered expression (3fold difference, present call rate of 75%, p < 0.05) and 7 of these had a connection to HIV-1 pathogenesis. In 4 genes: TOP1, CXCR2, SREBF2, and TAP we found a different regulation which was consistent with a supposed beneficial effect for CCR5-delta32 heterozygotes. Conclusion The CCR5-delta32 deletion is associated with other HDFs in HIV-1 pathogenesis as a possible explanation for beneficial effects regarding the deletion leading to a variant expression profile in heterozygous carriers of this mutation.
- Subjects
INFECTIOUS disease transmission; GENE expression; GENES; HIV; HIV infections; POLYMERASE chain reaction; RESEARCH funding; U-statistics; DISEASE progression; DESCRIPTIVE statistics
- Publication
AIDS Research & Therapy, 2013, Vol 10, p26
- ISSN
1742-6405
- Publication type
Article
- DOI
10.1186/1742-6405-10-26