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- Title
Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells.
- Authors
Wichit, Sineewanlaya; Jittmittraphap, Akanitt; Hidari, Kazuya I.P.J.; Thaisomboonsuk, Butsaya; Petmitr, Songsak; Ubol, Sukathida; Aoki, Chie; Itonori, Saki; Morita, Koichi; Suzuki, Takashi; Suzuki, Yasuo; Jampangern, Wipawee
- Abstract
Dengue viruses infect cells by attaching to a surface receptor which remains unknown. The putative receptor molecules of dengue virus type 2 on the surface of mosquito (AP-61) and mammalian (LLC-MK2) cell lines were investigated. The immunochemical detection and structural analysis of carbohydrates demonstrated that the neutral glycosphingolipids, L-3 (GlcNAcβ1-3Manβ1-4Glcβ1-1'Cer) in AP-61 cells, and nLcCer (Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer) in LLC-MK2 cells were recognized by the virus. These findings strongly suggest that neutral glycosphingolipids share the key determinant for virus binding and that the β-GlcNAc residue may play an important role in dengue virus binding to the host cell surface.
- Subjects
DENGUE viruses; CARBOHYDRATES; GLYCOSPHINGOLIPIDS; MAMMALS; MOSQUITOES; CYTOLOGY; CELL membranes; STRUCTURAL analysis (Science)
- Publication
Microbiology & Immunology, 2011, Vol 55, Issue 2, p135
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/j.1348-0421.2010.00293.x