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- Title
Characterization of an insect-specific flavivirus (OCFV<sub>PT</sub>) co-isolated from <i>Ochlerotatus caspius</i> collected in southern Portugal along with a putative new Negev-like virus.
- Authors
Ferreira, Daniela Duque; Cook, Shelley; Lopes, Ângela; de Matos, António Pedro; Esteves, Aida; Abecasis, Ana; de Almeida, António Paulo Gouveia; Piedade, João; Parreira, Ricardo
- Abstract
We describe the isolation and characterization of an insect-specific flavivirus (ISF) from Ochlerotatuscaspius (Pallas, 1771) mosquitoes collected in southern Portugal. The RNA genome of this virus, tentatively designated OCFV PT, for O. caspius flavivirus from Portugal, encodes a polyprotein showing all the features expected for a flavivirus. As frequently observed for ISF, the viral genomes seems to encode a putative Fairly Interesting Flavivirus ORF (FIFO)-like product, the synthesis of which would occur as a result of a −1 translation frameshift event. OCFV PT was isolated in the C6/36 Stegomyia albopicta (= Aedes albopictus) cell line where it replicates rapidly, but failed to replicate in Vero cells in common with other ISFs. Unlike some of the latter, however, the OCFV PT genome does not seem to be integrated in the mosquito cells we tested. Phylogenetic analyses based on partial ISF NS5 nucleotide sequences placed OCFV PT among recently published viral strains documented from mosquitoes collected in the Iberian Peninsula, while analyses of ORF/E/NS3/or NS5 amino acid sequences cluster OCFV PT with HANKV (Hanko virus), an ISF recently isolated from O. caspius mosquitoes collected in Finland. Taking into account the genetic relatedness with this virus, OCFV PT is not expected to be overtly cytopathic to C6/36 cells. The cytopathic effects associated with its presence in culture supernatants are postulated to be the result of the replication of a co-isolated putative new Negev-like virus.
- Subjects
FLAVIVIRUSES; FLAVIVIRAL diseases; RNA viruses; VIRAL genomes; MICROBIAL genomes
- Publication
Virus Genes, 2013, Vol 47, Issue 3, p532
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-013-0960-9