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- Title
A novel active pentapeptide from chicken brain identified by antibodies to FMRFamide.
- Authors
Dockray, G. J.; Reeve, J. R.; Shively, J.; Gayton, R. J.; Barnard, C. S.
- Abstract
The tetrapeptide Phe-Met-Arg-Phe-NH2 (FMRFamide) and peptides structurally related to it, have been isolated from molluscan ganglia1-3. They have widespread actions on both invertebrate and vertebrate tissues and there is increasing evidence that they are an important group of invertebrate peptide neurotransmitters3-8. It is of interest that the primary amino acid sequence of FMRFamide forms the C-terminal tetrapeptide of an enkephalin-like heptapeptide (Met-enkephalin-Arg6Phe7) isolated from bovine adrenal medulla and striatum9,10. Antisera to FMRFamide have been shown to react in radioimmunoassay and immunohistochemistry with material in the central nervous system of various vertebrate species11-17, but the identity of this material, and in particular its relationship to the opioid heptapeptide, remains uncertain. We have used antibodies specific for the C-terminus of FMRFamide in radioimmunoassays to monitor purification of the material in chicken brain. We describe here the sequence of one of the peptides obtained. It is a biologically active peptide which does not seem to be related to other known vertebrate neuropeptides.
- Publication
Nature, 1983, Vol 305, Issue 5932, p328
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/305328a0