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- Title
Characterization and application of keratinase and protease from hog hair degradation strains.
- Authors
Zhuang, Z. J.; Chang, K. C.; Cheng, Y. H.; Yu, Y. H.; Chen, W. J.
- Abstract
Hog hair is composed of keratin, an insoluble structural protein. It is barely hydrolyzed by common proteases. In this study, we intended to isolate and characterize several hog hair degradation strains from by-products to deal with the problem of hog hair waste. We cultured several Bacillus strains by using hog hair as sole carbon and nitrogen source. Then, the culture medium was centrifuged and the suspension was collected as crude enzyme (CE). The protease activity CE Could be confirmed by well difussion assay and zymogram analysis. Among them, CE from Bacillus licheniformis showed the highest protease activity (92 U/ml) at 60 C, pH7. Protease inhibitor assay confirmed that the CE is Mainly consisted of serine proteases. Currently, we are measuring the weight loss percentage of hog hair, the increased quantity of soluble protein and the content of essential amino acids after hydrolyzing hog hair by CE, and evaluate their potential of serving as animal and plant feed additives.
- Subjects
PROTEOLYTIC enzymes; KERATIN; CYTOSKELETAL proteins; BACILLUS (Bacteria); PROTEASE inhibitors
- Publication
Journal of the Chinese Society of Animal Science, 2019, Vol 48, p329
- ISSN
0253-9187
- Publication type
Article