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- Title
Optimization of a fermentation medium for the production of Penicillin G acylase from Bacillus sp.
- Authors
Rajendhran, J.; Krishnakumar, V.; Gunasekaran, P.
- Abstract
Aims: Optimization of Penicillin G acylase (PAC) production from a novel isolate of Bacillus sp. Methods: Fermentation medium for PAC production was optimized using a two-level fractional factorial design with seven components. Results: A maximum production of 9·5 U ml-1 of PAC was obtained in an optimized medium containing (g l-1 ): K2 HPO4 , 1·0; MgSO4 ·7H2 O, 0·1; CaCl2 ·2H2 O, 0·1; PAA, 2·0; tryptone, 5·0; yeast extract, 3·0; and sucrose, 50·0. Significance and Impact of the Study: The two-step medium optimization resulted in a twofold increase in PAC production. Since the strain Bacillus sp. PGS10 produces a high level of PAC, it could be a potential candidate for industrial production of PAC.
- Subjects
PENICILLIN; BACILLUS sphaericus; FERMENTATION
- Publication
Letters in Applied Microbiology, 2002, Vol 35, Issue 6, p523
- ISSN
0266-8254
- Publication type
Article
- DOI
10.1046/j.1472-765X.2002.01234.x