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- Title
Accumulation of low-dose BIX01294 promotes metastatic potential of U251 glioblastoma cells.
- Authors
MIN YOUNG KIM; SHIN-JI PARK; JAE WOONG SHIM; YU JIN SONG; KWANGMO YANG; SEONG-JOON PARK; KYU HEO
- Abstract
BIX01294 (Bix) is known to be a euchromatic histone-lysine N-methyltransferase 2 inhibitor and treatment with Bix suppresses cancer cell survival and proliferation. In the present study, it was observed that sequential treatment with low-dose Bix notably increases glioblastoma cell migration and metastasis. It was demonstrated that U251 cells sequentially treated with low-dose Bix exhibited induced characteristic changes in critical epithelial-mesenchymal transition (EMT) markers, including E-cadherin, N-cadherin, β-catenin and zinc finger protein SNAI2. Notably, sequential treatment with Bix also increased the expression of cancer stem cell-associated markers, including sex determining region Y-box 2, octamer-binding transcription factor 4 and cluster of differentiation 133. Neurosphere formation was significantly enhanced in cells sequentially treated with Bix, compared with control cells (control: P=0.011; single treatment of Bix, P=0.045). The results of the present study suggest that accumulation of low-dose Bix enhanced the migration and metastatic potential of glioblastoma cells by regulating EMT-associated gene expression, which may be the cause of the altered properties of glioblastoma stem cells.
- Subjects
HISTONE methyltransferases; GLIOBLASTOMA multiforme treatment; ZINC-finger proteins; TRANSCRIPTION factors; CELL differentiation
- Publication
Oncology Letters, 2017, Vol 13, Issue 3, p1767
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2017.5626