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- Title
Single‐cell atlas of peripheral blood mononuclear cells from pregnant women.
- Authors
Chen, Dongsheng; Wang, Wei; Wu, Linlin; Liang, Langchao; Wang, Shiyou; Cheng, Yunfeng; Zhang, Tongda; Chai, Chaochao; Luo, Qiuhong; Sun, Chengcheng; Zhao, Wandong; Lv, Zhiyuan; Gao, Ya; Wu, Xiaoxia; Sun, Ning; Zhang, Yiwei; Zhang, Jing; Chen, Yixuan; Tong, Jianing; Wang, Xiangdong
- Abstract
Background: During pregnancy, mother–child interactions trigger a variety of subtle changes in the maternal body, which may be reflected in the status of peripheral blood mononuclear cells (PBMCs). Although these cells are easy to access and monitor, a PBMC atlas for pregnant women has not yet been constructed. Methods: We applied single‐cell RNA sequencing (scRNA‐seq) to profile 198,356 PBMCs derived from 136 pregnant women (gestation weeks 6 to 40) and a control cohort. We also used scRNA‐seq data to establish a transcriptomic clock and thereby predicted the gestational age of normal pregnancy. Results: We identified reconfiguration of the peripheral immune cell phenotype during pregnancy, including interferon‐stimulated gene upregulation, activation of RNA splicing‐related pathways and immune activity of cell subpopulations. We also developed a cell‐type‐specific model to predict gestational age of normal pregnancy. Conclusions: We constructed a single‐cell atlas of PBMCs in pregnant women spanning the entire gestation period, which should help improve our understanding of PBMC composition turnover in pregnant women.
- Subjects
MONONUCLEAR leukocytes; PREGNANT women; MOTHER-child relationship
- Publication
Clinical & Translational Medicine, 2022, Vol 12, Issue 5, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.821