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- Title
Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach.
- Authors
Sun, Y.; Li, J.-Y.; He, J.-S.; Zhou, L.-X.; Chen, K.
- Abstract
Sun Y, Li J-Y, He J-S, Zhou L-X&Chen K(2005)Histopathology46,505–514Tissue microarray analysis of multiple gene expression in intestinal metaplasia, dysplasia and carcinoma of the stomach: To study multiple gene expression patterns and their roles in the process of gastric carcinogenesis.: Using a high-throughput tissue microarray technique, 169 specimens from gastric carcinomas, precursor lesions and normal mucosa were immunostained on a series of tissue chips for p53, p21WAF1/CIP1 cyclin E, Bcl-2, c-met and mucin 5AC expression. The overexpression of p53 was observed in 10.7% of low-grade dysplasia (LGD), 38.1% of high-grade dysplasia (HGD) and 39.6% of intestinal type gastric carcinoma (IGC). Expression of p21WAF1/CIP1 was found in 47.6% of incomplete intestinal metaplasia (IM), 36.7% of dysplasia (Dys) and 29.5% of IGC. The overexpression of cyclin E was more frequently present in carcinomas than in Dys (P < 0.05); moreover, high-level expression (>25% in extent) of cyclin E was observed only among IGC. Abnormal Bcl-2 expression was present in 81.0% of incomplete IM, 69.4% of Dys and 22.9% of IGC. Along with progression of the lesion, the expression of c-met increased; in contrast, mucin 5AC decreased gradually.: The specific expression pattern in incomplete IM was mucin 5AC(+)/Bcl-2(+)/p53(–)/cyclin E(–), while mucin 5AC(–)/cyclin E(+) was specific for IGC. p53 was useful for distinguishing LGD from HGD. High-level expression of cyclin E might be an indicator for malignant transformation of dysplasia.
- Subjects
CANCER; GENE expression; DNA microarrays; DYSPLASIA; MUCINS; CARCINOGENESIS
- Publication
Histopathology, 2005, Vol 46, Issue 5, p505
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2005.02111.x