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- Title
Catechins inhibit vascular endothelial growth factor production and cyclooxygenase-2 expression in human dental pulp cells.
- Authors
Nakanishi, T.; Mukai, K.; Hosokawa, Y.; Takegawa, D.; Matsuo, T.
- Abstract
Aim To investigate the effect of catechins on vascular endothelial growth factor ( VEGF) production and cyclooxygenase-2 ( COX-2) expression in human dental pulp cells ( HDPC) stimulated with bacteria-derived factors or pro-inflammatory cytokines. Methodology Morphologically fibroblastic cells established from explant cultures of healthy human dental pulp tissues were used as HDPC. HDPC pre-treated with catechins, epigallocatechin-3-gallate ( EGCG) or epicatechin gallate ( ECG), were exposed to lipopolysaccharide ( LPS), peptidoglycan ( PG), interlukin-1β ( IL-1β) or tumour necrosis factor-α ( TNF-α). VEGF production was examined by enzyme-linked immunosorbent assay, and COX-2 expression was assessed by immunoblot. Results EGCG and ECG significantly reduced LPS- or PG-mediated VEGF production in the HDPC in a dose-dependent manner. EGCG also prevented IL-1β-mediated VEGF production. Although TNF-α did not enhance VEGF production in the dental pulp cells, treatment of 20 μg mL−1 of EGCG decreased the level of VEGF. In addition, the catechins attenuated COX-2 expression induced by LPS and IL-1β. Conclusions The up-regulated VEGF and COX-2 expressions in the HDPC stimulated with these bacteria-derived factors or IL-1β were diminished by the treatment of EGCG and ECG. These findings suggest that the catechins may be beneficial as an anti-inflammatory tool of the treatment for pulpal inflammation.
- Subjects
CATECHIN; VASCULAR endothelial growth factors; CYCLOOXYGENASE 2; DENTAL pulp; CYTOKINES; FIBROBLASTS; TUMOR necrosis factors; ENZYME-linked immunosorbent assay
- Publication
International Endodontic Journal, 2015, Vol 48, Issue 3, p277
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/iej.12312