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- Title
Ultra-high-field MRI real-time imaging of HSC engraftment of the bone marrow niche.
- Authors
Bengtsson, N. E.; Kim, S.; Lin, L.; Walter, G. A.; Scott, E. W.
- Abstract
The bone marrow (BM) undergoes extensive remodeling following irradiation damage. A crucial part of restoring homeostasis following irradiation is the ability of hematopoietic stem cells (HSCs) to home to and engraft specialized niches within the BM through a remodeling BM vascular system. Here we show that a combination of ultra-high-field strength magnetic resonance imaging (17.6 T, MRI) coupled with fluorescent microscopy (FLM) serves as a powerful tool for the in vivo imaging of cell homing within the BM. Ultra-high-field MRI can achieve high-resolution three-dimensional (3D) images (28 × 28 × 60 μm(3)) of the BM in live mice, sufficient to resolve anatomical changes in BM microstructures attributed to radiation damage. Following intra-arterial infusion with dsRed-expressing BM cells, labeled with superparamagnetic iron oxides, both FLM and MRI could be used to follow initial homing and engraftment of donor HSC to a limited number of preferred sites within a few cell diameters of the calcified bone-the endosteal niche. Subsequent histology confirmed the fidelity and accuracy of MRI to create non-invasive, high-resolution 3D images of donor cell engraftment of the BM in living animals at the level of single-cell detection.
- Subjects
BONE marrow; IRRADIATION; HOMEOSTASIS; HEMATOPOIETIC stem cells; BLOOD vessels; FLUORESCENCE microscopy; LABORATORY mice
- Publication
Leukemia (08876924), 2011, Vol 25, Issue 8, p1223
- ISSN
0887-6924
- Publication type
journal article
- DOI
10.1038/leu.2011.72