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- Title
Co<sub>3</sub>O<sub>4</sub>/PAn MAGNETIC NANOPARTICLE-MODIFIED ELECTROCHEMICAL IMMUNOSENSOR FOR CHLORPYRIFOS.
- Authors
WANG, W. H.; HAN, Z. J.; LIANG, P. J.; GUO, D. Q.; XIANG, Y. J.; TIAN, M. X.; SONG, Z. L.; ZHAO, H. R.
- Abstract
In this study, Co3O4/PAn nanoparticles modified electrochemical immunosensor was produced, and applied to rapidly detect chlorpyrifos residues in agricultural products. Chlorpyrifos(CPF)-BSA artificial antigen was coupled to the surface of working electrodes coated with the Co3O4/PAn nanoparticles thin layer. The competitive reaction is launched between the CPF in the samples and the CPF coupled to the electrodes with CPF monoclonal antibodies in the test system. After the antigen-antibody signal is amplified by silver nanoparticles, the electrolytic current under a certern voltage is detected, which was called as voltammetry. After establishing basic detection system, a series of indices including the concentration of immobilized membrane, materials coated on the electrode surface, buffer, the concentration of coupled antigen and secondary antibodies were optimized. Then the concentration of CPF in standands, green vegetables and apples was detected. The results show that the immune sensor has good sensitivity and high accuracy; Lower Limit of Detection (LLD) is 0.01μg/mL for CPF, recovery rate is more than 82%, test time is less than 2 h, and variable coefficient is less than 5%. The sensor is economical because it can be reused after reusing treatment. The study contributes to realize rapid detection of pesticide residues in agricultural products.
- Subjects
MAGNETIC nanoparticles; NANOSTRUCTURED materials synthesis; COBALT oxides; ELECTROCHEMICAL analysis; CHLORPYRIFOS; BIOSENSORS; SILVER nanoparticles; ELECTRODES
- Publication
Digest Journal of Nanomaterials & Biostructures (DJNB), 2017, Vol 12, Issue 1, p1
- ISSN
1842-3582
- Publication type
Article