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- Title
Consolidated bioprocessing of butanol production from xylan by a thermophilic and butanologenic <italic>Thermoanaerobacterium</italic> sp. M5.
- Authors
Jiang, Yujia; Guo, Dong; Lu, Jiasheng; Dürre, Peter; Dong, Weiliang; Yan, Wei; Zhang, Wenming; Ma, Jiangfeng; Jiang, Min; Xin, Fengxue
- Abstract
Background: Consolidated bioprocessing (CBP) has attracted increasing attention since it can accomplish hydrolytic enzymes production, lignocellulose degradation and microbial fermentation in one single step. Currently, biobutanol is mainly produced by mesophilic and solventogenic clostridia, such as <italic>Clostridium beijerinckii</italic> and <italic>C. acetobutylicum</italic>, which cannot directly utilize lignocellulose, an abundant, renewable and economic feedstock. Hence, metabolic construction or isolation of novel cellulolytic/hemicellulolytic and solventogenic bacteria to achieve direct butanol production from lignocellulose offers a promising alternative. Results: In this study, a newly isolated <italic>Thermoanaerobacterium</italic> sp. M5 could directly produce butanol from xylan through CBP at 55 °C via the butanol–ethanol pathway. Further genomic and proteomic analysis showed that the capabilities of efficient xylan degradation and butanol synthesis were attributed to the efficient expression of xylanase, β-xylosidase and the bifunctional alcohol/aldehyde dehydrogenase (AdhE). Process optimization based on the characteristic of AdhE could further improve the final butanol titer to 1.17 g/L from xylan through CBP. Furthermore, a new co-cultivation system consisting of <italic>Thermoanaerobacterium</italic> sp. M5 which could release xylose from xylan efficiently and <italic>C. acetobutylicum</italic> NJ4 which possesses the capacity of high butanol production was established. This microbial co-cultivation system could improve the butanol titer to 8.34 g/L, representing the highest butanol titer from xylan through CBP. Conclusions: A newly thermophilic and butanogenic bacterium <italic>Thermoanaerobacterium</italic> sp. M5 was isolated and key enzymes responsible for butanol production were characterized in this study. High butanol titer was obtained from xylan through process optimization. In addition, the newly set up microbial co-cultivation system, consisting of <italic>Thermoanaerobacterium</italic> sp. M5 and <italic>C. acetobutylicum</italic> NJ4, achieved the highest butanol production from xylan compared with the reported co-cultivation systems.
- Subjects
BIOMASS energy; THERMOPHILIC microorganisms; BUTANOL; HYDROGEN production; BIOMASS; MICROBIOLOGICAL synthesis; POLYSACCHARIDES
- Publication
Biotechnology for Biofuels, 2018, Vol 11, p1
- ISSN
1754-6834
- Publication type
Article
- DOI
10.1186/s13068-018-1092-1