We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Preparation of Rat Whole-kidney Acellular Matrix via Peristaltic Pump.
- Authors
Liu, Rongfu; Gao, Jiasheng; Yang, Yufeng; Zeng, Weixin; Liu, Rong; Gao, Jia; Yang, Yu; Zeng, Wei; Liu, Rong Fu; Gao, Jia sheng; Yang, Yu Feng; Zeng, Wei Xin
- Abstract
<bold>Purpose: </bold>To design a whole-kidney a cellular matrix scaffold using peristaltic pump perfusion and to ascertain the retention of extra cellular proteins by the scaffold.<bold>Materials and Methods: </bold>Male Sprague-Dawley (SD) rats weighing 200-250 g were used. Intravenous catheters were inserted into the renal artery followed by perfusion of decellularization solution using a peristaltic pump. After decellularization, the acellular matrix was observed under a microscope after hematoxylin and eosin (H&E) staining and a fluorescence microscope after 4′,6-diamidino-2-phenylindole (DAPI) staining. Immunohistochemistry was used to identify the composition of kidney acellular matrix.<bold>Results: </bold>The result of H&E and DAPI staining demonstrate the removal of cellular material in kidney a cellular matrix. Immunohistochemistry confirmed the conservation of the natural expression of extra cellular matrix proteins including collagen types I and IV, fibrin and laminin.<bold>Conclusion: </bold>Peristaltic pump perfusion enables successful preparation of renal a cellular matrix, to retainthe criticalproteins of natural extra cellular matrix. The resulting kidney a cellular matrix represents an ideal natural scaffold for renal tissue engineering.
- Subjects
ANIMALS; BIOMEDICAL materials; CELL separation; COLLAGEN; CULTURE media (Biology); EXTRACELLULAR space; FIBRIN; IMMUNOHISTOCHEMISTRY; KIDNEYS; MEMBRANE proteins; PERFUSION; RATS; STAINS &; staining (Microscopy); TISSUE engineering
- Publication
Urology Journal, 2015, Vol 12, Issue 6, p2457
- ISSN
1735-1308
- Publication type
journal article