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- Title
Enhancement of antitumor immunity by prolonging antigen presentation on dendritic cells.
- Authors
Wang, Rong-Fu; Wang, Helen Y.
- Abstract
Vaccination with dendritic cells (DCs) pulsed with antigenic peptides derived from various tumor antigens has great, but as yet significantly unrealized, potential in cancer treatment. Here, we describe a strategy for prolonged presentation of an MHC class I-restricted self-peptide on DCs through linkage of it to a cell penetrating peptide (CPP). DCs loaded with a peptide derived from tyrosinase-related protein 2 (TRP2) covalently linked to a CPP1 sequence retained full capacity to stimulate T cells for at least 24 h, completely protected immunized mice from subsequent tumor challenge, and significantly inhibited lung metastases in a 3-day tumor model. DCs pulsed with TRP2 alone failed to provide any of these protections. In addition, we demonstrate that both CD4+ and CD8+ T cells were required for potent antitumor immunity. This CPPbased approach may be generally applicable to enhance the efficacy of DC-based peptide vaccines against cancer and other diseases.
- Subjects
CANCER vaccines; DENDRITIC cells
- Publication
Nature Biotechnology, 2002, Vol 20, Issue 2, p149
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/nbt0202-149